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An inverted microscope is specifically designed with the objectives positioned beneath the stage, which is the characteristic feature that distinguishes it from standard upright microscopes. This configuration allows for greater accessibility to the specimen from above, facilitating the observation of samples in larger containers such as Petri dishes or culture flasks. The unique setup is particularly advantageous for examining live cells, as it enables easy manipulation and viewing without the need to transfer samples to a traditional slide.

Although inverted microscopes may have built-in light sources and can be used for live cell imaging, these features are not exclusive to inverted microscopes and can also be found in other types of microscopes. Additionally, while some inverted microscopes may offer high magnification, the characteristic feature that critically defines their design is the location of the objectives underneath the stage.